Catalog Number - 59518-25P

MicroBee C18™ is a traditional octadecylsilane (C18) reversed-phase HPLC stationary phase that has been trusted by chromatographers for many years. As part of the Cogent™ Similars product line, it provides a cost-effective alternative for established analytical methods while delivering the hydrophobic retention characteristics expected from classical C18 chemistry. The end-capped, Type-A silica support is bonded with a straight-chain C18 ligand, making it particularly useful for laboratories operating legacy methods and compendial procedures.

59518-25P is a 250 mm x 4.6 mm ID analytical column packed with 5 µm, 125Å MicroBee C18 particles. The 250 mm length provides increased resolving power and higher theoretical plate counts compared to shorter columns, making it well suited for impurity profiling, complex sample separations, and methods requiring greater chromatographic resolution. The 5 µm particle size delivers improved efficiency and sharper peak shapes than 10 µm packings, while the industry-standard 4.6 mm ID format typically operates optimally at flow rates of approximately 1.0–2.0 mL/min on conventional HPLC systems.

This USP L1 column is an excellent alternative for methods developed on Microbondapak™ and similar C18 columns. The traditional C18 chemistry is ideal for hydrophobic and neutral compounds analyzed under reversed-phase conditions and is particularly effective for long-chain fatty acids, pharmaceutical compounds, and other lipophilic analytes. The combination of higher efficiency and increased column length makes this configuration a strong choice when additional separation is required without moving to smaller particle technologies.

Key Benefits

• Traditional end-capped C18 stationary phase

• Suitable alternative for USP L1 methods

• Compatible with many legacy Microbondapak™ methods

• Higher efficiency with 5 µm particles

• Increased resolution from 250 mm column length

• Improved peak shape and chromatographic performance

• Excellent for hydrophobic and neutral compounds

• Cost-effective Cogent Similars column

Typical Applications

• USP L1 methods

• Reversed-phase HPLC

• Pharmaceutical analysis

• Quality control testing

• Method development

• Impurity profiling

• Long-chain fatty acid analysis

• Lipophilic compound separations

Specifications

• Product Type: HPLC Column

• Stationary Phase: MicroBee C18

• USP Classification: L1

• Particle Size: 5 µm

• Particle Type: Type-A Silica

• Bonded Phase: C18 (Octadecylsilane)

• Endcapped: Yes

• Pore Size: 125Å

• Column Dimensions: 4.6 mm ID x 250 mm

• Length: 250 mm

• Recommended Flow Rate: 1.0–2.0 mL/min

• Brand: Cogent

Important Notes

• Designed for reversed-phase chromatography

• Traditional end-capped C18 chemistry

• 5 µm particles provide higher efficiency and sharper peaks

• Compatible with conventional HPLC systems

• Industry-standard 4.6 mm ID analytical format

• Longer column length provides increased resolving power

• Excellent alternative for many USP L1 methods

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